| Wild 'Til 9 Podcast Series | 2022 | Actress | Guest |
| Talking Tom and Friends | 2021 | Actress | Rhonda |
| You Know Me... Now Meet My Pet TV Series | 2019 | Actress | |
| Go Fish | 2019 | Actress | Callie (voice, as iJustine) |
| Escape the Night | 2016–2019 | Actress | The Adventurer |
| Ask Orange | 2011–2019 | Actress | Passion Fruit (voice) |
| The Annoying Orange | 2010–2018 | Actress | Passion Fruit |
| Alex Clark: Animated Storytimes TV Series | 2017 | Actress | Nurse |
| My Babysitter Story TV Series | 2017 | Actress | Nurse (voice) |
| Sugar Pine 7 | 2017 | Actress | Justine |
| Little Bird's Big Adventure | 2017 | Actress | Social Media Pigeons (voice) |
| Minute Match-Ups | 2016 | Actress | Princess Allura |
| Sharknado 4: The 4th Awakens | 2016 | Actress | Shatner |
| Bad Internet | 2016 | Actress | LizzieBeth |
| Air Bound | 2015 | Actress | Shelly (English version, voice) |
| Lazer Team | 2015 | Actress | Vlogger |
| Fight of the Living Dead | 2015 | Actress | Contestant |
| Rosanna Pansino: Perfect Together | 2015 | Actress | |
| The Wedding Ringer | 2015 | Actress | Stuart's Wife Pam |
| Annoying Orange Holiday Fruitacular | 2014 | Actress | Passion Fruit (voice) |
| Disconnected Short | 2014 | Actress | Sophie (uncredited) |
| The Hunger Games: Mockingjay - District Voices | 2014 | Actress | Justine Ezarik (uncredited) |
| The High Fructose Adventures of Annoying Orange | 2012–2014 | Actress | Passion Fruit |
| Lauren | 2013 | Actress | Annie |
| MyMusic | 2012–2013 | Actress | Hipster Idol |
| VGHS: The Movie | 2012 | Actress | Rosalie |
| Rewind YouTube Style 2012 | 2012 | Actress | Girl playing violin (as iJustine) |
| The Guild | 2012 | Actress | Sabina |
| Video Game High School | 2012 | Actress | Bella |
| The Vampire Diaries | 2012 | Actress | Pretty Bartender |
| The Street Fighter | 2011 | Actress | Rebecca |
| Minecraft Gets Played | 2011 | Actress | Justine |
| Last Moments of Relationships | 2011 | Actress | Girlfriend |
| The Bold and the Beautiful | 2010 | Actress | Reporter #3 |
| Criminal Minds | 2010 | Actress | Meredith Joy |
| Level 26: Dark Prophecy | 2010 | Actress | Kate Hale |
| BlackBoxTV | 2010 | Actress | Woman (as iJustine) |
| The House That Drips Blood on Alex | 2010 | Actress | Melissa |
| YouTube Assassin | 2010 | Actress | Justine Ezarik |
| Dropout | 2009 | Actress | Vlogger (as iJustine) |
| Totally Sketch | 2009 | Actress | iJustine |
| Law & Order: Special Victims Unit | 2009 | Actress | A.J. Dunne |
| The Station | 2009 | Actress | iJustine |
| A Brave Heart: The Lizzie Velasquez Story | 2015 | Producer | executive producer |
| Stare Into the Lights My Pretties | 2017 | Camera and Electrical Department | additional photography |
| The New Corporation: The Unfortunately Necessary Sequel | 2020 | Additional Crew | additional material provided by (as IJustine) |
| The Gauntlet | 2013 | Additional Crew | squad member: Internet Celebrity |
| Physics Girl | 2019 | Thanks | many thanks to |
| Rien de 9 | 2010 | Thanks | special thanks |
| The Game Awards 2023 | 2023 | Self | Self - Audience Member |
| Blindspots TV Series | 2023 | Self | Self |
| Impact Video | 2021 | Self | Self |
| So, I Made a Decision... | 2020 | Self | Self |
| Animal Talking with Gary Whitta | 2020 | Self | Self - Guest |
| MrBeast | 2020 | Self | Self (as iJustine) |
| Ro's Life | 2016–2020 | Self | Self |
| Retro Tech | 2019 | Self | Self |
| Good Morning America | 2019 | Self | Self - Guest |
| Physics Girl | 2019 | Self | Self (as iJustine) |
| YouTubers React | 2012–2018 | Self | Self |
| 50 Amazing Facts to Blow Your Mind | 2018 | Self | Self |
| Fortnite Celebrity Pro-AM | 2018 | Self | Self (host) |
| Nerdy Nummies | 2017–2018 | Self | Self |
| YouTube Rewind: The Shape of 2017 | 2017 | Self | Self (as iJustine) |
| CODumentary | 2017 | Self | Self |
| Live with Kelly and Mark | 2017 | Self | Self - Guest (as iJustine) |
| Good Mythical Morning | 2017 | Self | Self |
| Steve Harvey | 2017 | Self | Self |
| Home & Family | 2017 | Self | Self |
| Brave Enough | 2017 | Self | Self |
| Unzipped with Lizzie Velasquez TV Series | 2017 | Self | Self |
| Chopped | 2017 | Self | Self - Contestant |
| The Apprentice | 2017 | Self | Self - Boardroom Advisor |
| YouTube Rewind: The Ultimate 2016 Challenge | 2016 | Self | Self (as iJustine) |
| Chopped Junior | 2016 | Self | Self - Judge (as iJustine) |
| Annoying Orange HOW2 | 2016 | Self | Self |
| Casey Neistat Vlog | 2016 | Self | Self |
| Vlogumentary | 2016 | Self | Self |
| Prank Academy | 2016 | Self | Self |
| Red Carpet Report | 2016 | Self | Self |
| How to Motion Capture with iJustine Video | 2015 | Self | Self |
| Call of Duty: Black Ops 3 - Celebrity Zombie Auditions | 2015 | Self | Self |
| Movie3Some | 2015 | Self | Self |
| Geeks Who Drink | 2015 | Self | Self - Team Captain (as iJustine) |
| Batman: Arkham Knight - Classified First Look Video | 2015 | Self | Self |
| Pop Culture Underground | 2015 | Self | Self |
| Joey Graceffa | 2015 | Self | Self (as iJustine) |
| The World Dog Awards | 2015 | Self | Self - Presenter (as iJustine) |
| YouTube Rewind: Turn Down for 2014 | 2014 | Self | Self (as iJustine) |
| Drama King | 2014 | Self | Self |
| The Talk | 2014 | Self | Self - Guest (as iJustine) |
| Star Truk TV Series | 2014 | Self | Justine Ezarik |
| The Marshmallow Show TV Series | 2014 | Self | Self |
| Lamarr Wilson TV Series | 2014 | Self | Self (as iJustine) |
| TakePart Live | 2014 | Self | Self - Guest (as iJustine) |
| Jess in Distress! TV Series | 2014 | Self | Self |
| Takei's Take TV Series | 2014 | Self | Self |
| In Bed with Joan | 2014 | Self | Self - Guest |
| Piper's Picks TV | 2013 | Self | Self - Guest |
| Pancake Manor | 2013 | Self | Self |
| mental_floss: The List Show | 2013 | Self | Self - Host |
| Xbox One: Day One Countdown TV Special | 2013 | Self | Self |
| The Gauntlet | 2013 | Self | Self |
| RT Life TV Series | 2013 | Self | Self |
| The Rooster Teeth Podcast | 2013 | Self | Self |
| Grace's Faces TV Series | 2013–2014 | Self | Self |
| HardWired with iJustine | 2013 | Self | Self |
| VGA 10 All Access Live TV Special | 2012 | Self | |
| GTTV Live: Call of Duty - Black Ops II World Launch TV Special | 2012 | Self | |
| Call of Duty ELITE: Friday Night Fights | 2011–2012 | Self | Self - Host |
| Escape Routes | 2012 | Self | Self - Host |
| The Tommy Wi-Show | 2012 | Self | Self |
| Those Video Guys | 2011 | Self | iJustine |
| Vsauce | 2011 | Self | Self |
| GameTrailers TV with Geoff Keighley | 2011 | Self | Self |
| What's Trending | 2011–2014 | Self | Self |
| Rachael Ray | 2010 | Self | Self (uncredited) |
| The Price is Right | 2009 | Self | Self - Contestant |
| MTV Video Music Awards 2009 Pre-Show TV Special | 2009 | Self | Self - Host |
| InfoMania | 2009 | Self | Self |
| The Rotten Tomatoes Show | 2009 | Self | Self |
| Internet Superstar TV Series | 2008 | Self | Self - Guest |
| The Dark, Sad Life of Boogie2988 | 2023 | Archive Footage | Self (archive footage) |
| Chazington | 2022 | Archive Footage | Passionfruit (archive footage) |
| Sugar Pine 7 | 2017 | Archive Footage | Justine (archive footage) |
| Annoying Orange: Movie Fruitacular | 2014 | Archive Footage | Passion Fruit (archive footage) |
| IGN Daily Fix | 2012 | Archive Footage | Self (archive footage) |
| Vlogbrothers | 2011 | Archive Footage | Self (archive footage, uncredited) |
| Rien de 9 | 2010 | Archive Footage | Justine (archive footage) |