| The Standups | 2021 | Writer | Writer |
| Nuclear Family | 2016 | Writer | Writer (as Melissa Villasenor) |
| Funny as Hell | 2012 | Writer | writer (as Melissa Villasenor) |
| Love, Death & Robots | 2025 | Actress | Thermostat (voice) |
| Krapopolis | 2025 | Actress | Helen (voice) |
| Primos | 2024–2025 | Actress | Nellie |
| Win or Lose | 2025 | Actress | Veronica (voice) |
| Max and the Midknights | 2024–2025 | Actress | Millie (voice) |
| Dog Man | 2025 | Actress | Realtor (voice) |
| Disney Jr.'s Ariel | 2024–2025 | Actress | Navi |
| Rock, Paper, Scissors | 2024 | Actress | Pencil |
| Mulligan | 2024 | Actress | (voice) |
| Adventure Time: Fionna & Cake | 2023 | Actress | Babette |
| Kung Fu Panda: The Dragon Knight | 2023 | Actress | Akna (voice) |
| Ten Year Old Tom | 2021–2023 | Actress | Kasey (voice) |
| Night Court | 2023 | Actress | Gabby |
| Batman: The Audio Adventures | 2021–2022 | Actress | Robin (voice) |
| Saturday Night Live | 2016–2022 | Actress | Various |
| Amphibia | 2021–2022 | Actress | Ally |
| The Chicken Squad | 2021–2022 | Actress | Wheeze |
| Star Trek: Prodigy | 2022 | Actress | Nandi |
| Q-Force | 2021 | Actress | (voice) |
| I Heart Arlo | 2021 | Actress | Lily (voice) |
| Duncanville | 2021 | Actress | (voice) |
| Ultra City Smiths | 2021 | Actress | Sister Mary Margaret |
| Jellystone | 2021 | Actress | Susan the Guitar (voice) |
| Archibald's Next Big Thing Is Here | 2021 | Actress | Hazel |
| Crank Yankers | 2021 | Actress | Jennifer Lopez |
| Summer Camp Island | 2020 | Actress | Psychic (voice) |
| Hubie Halloween | 2020 | Actress | Cat Owner Karen |
| Awkwafina Is Nora from Queens | 2020 | Actress | |
| OK K.O.! Let's Be Heroes | 2017–2019 | Actress | Potato |
| Toy Story 4 | 2019 | Actress | Karen Beverly (voice) |
| Saturday Night Live: Cut for Time | 2017–2019 | Actress | Student |
| American Dad! | 2018–2019 | Actress | Foreign Exchange Student |
| Ralph Breaks the Internet | 2018 | Actress | Taffyta (voice) |
| Barry | 2018 | Actress | Waitress |
| Alone Together | 2018 | Actress | Cammy (as Melissa Villasenor) |
| Crashing | 2018 | Actress | Melissa Villasenor (as Melissa Villasenor) |
| The David S. Pumpkins Halloween Special | 2017 | Actress | (voice, as Melissa Villasenor) |
| F Is for Family | 2017 | Actress | Claire (voice) |
| Scooby-Doo! Shaggy's Showdown | 2017 | Actress | Tawny Rogers (voice, as Melissa Villasenor) |
| Adventure Time | 2012–2017 | Actress | Additional Voices |
| Jeff & Some Aliens | 2017 | Actress | Julie |
| Doll Eyes Short | 2016 | Actress | Angela (as Melissa Villasenor) |
| Drive By Short | 2016 | Actress | Shana (as Melissa Villasenor) |
| Mary + Jane | 2016 | Actress | Erin (as Melissa Villasenor) |
| Nuclear Family | 2016 | Actress | Bethany Barker (as Melissa Villasenor) |
| Laid in America | 2016 | Actress | Ms. Hopkins (as Melissa Villasenor) |
| TripTank | 2014–2016 | Actress | Teeny Moodle |
| Bad Weather Films | 2014–2016 | Actress | Maggie (as Melissa Villasenor) |
| Pickle and Peanut | 2016 | Actress | Darla (voice, as Melissa Villasenor) |
| Last Moments of Relationships | 2016 | Actress | Britney Spears (as Melissa Villasenor) |
| Guber | 2016 | Actress | Lead (as Melissa Villasenor) |
| Impress Me | 2015 | Actress | Melissa Vilasenor |
| Command Center TV Mini Series | 2015 | Actress | Melissa (as Melissa Villasenor) |
| The Most Best Talk Show | 2015 | Actress | (as Melissa Villasenor) |
| 29 Celebrity Impressions, 1 Original Song | 2014 | Actress | Britney Spears |
| The Funtime Gang TV Movie | 2013 | Actress | Chelsea the Chicken (as Melissa Villasenor) |
| Hispanic Comedians: from Gabriel Iglesias to Melissa Villaseñor on growing up funny Video | 2012 | Actress | (as Melissa Villasenor) |
| Family Guy | 2012 | Actress | Nora Ephron (voice, as Melissa Villasenor) |
| Naked But Funny | 2010 | Actress | (as Melissa Villasenor) |
| The 41-Year-Old Virgin Who Knocked Up Sarah Marshall and Felt Superbad About It | 2010 | Actress | Sara (as Melissa Villasenor) |
| Battle Realms: Winter of the Wolf | 2002 | Actress | Yvaine (uncredited) |
| Jesus Trejo: Stay at Home Son | 2020 | Thanks | special thanks |
| Saturday Night Live | 2019–2022 | Soundtrack | performer: "The Lion Sleeps Tonight" |
| The Standups | 2021 | Soundtrack | performer: "Hopelessly Devoted to You", "How You Remind Me", "The Reason", "Chop Suey" (uncredited) |
| 29 Celebrity Impressions, 1 Original Song | 2014 | Soundtrack | performer: "Perfect" |
| SNL50: The Homecoming Concert | 2025 | Self | Self - Audience Member |
| 65th Annual L.A. County Holiday Celebration TV Special | 2024 | Self | Self |
| After Midnight | 2024 | Self | Self |
| The Late Show with Stephen Colbert | 2023 | Self | Self |
| Celebrity Wheel of Fortune | 2023 | Self | Self - Celebrity Contestant |
| Is It Cake? | 2023 | Self | Self - Judge |
| That's My Jam | 2022 | Self | Self |
| Reindeer in Here | 2022 | Self | Candy (voice) |
| The Drew Barrymore Show | 2022 | Self | Guest |
| GMA3: What You Need to Know | 2022 | Self | Self |
| The Kelly Clarkson Show | 2022 | Self | Self |
| Finding Your Roots with Henry Louis Gates, Jr. | 2022 | Self | Self |
| The Standups | 2021 | Self | Self |
| Lost Odyssey: Promised Gold Video | 2021 | Self | Self - Player |
| Saturday Night Live | 2021 | Self | Self |
| Glitter and Garbage Podcast Series | 2021 | Self | Self |
| 2021 Film Independent Spirit Awards | 2021 | Self | Host - Various |
| 36th Film Independent Spirit Awards TV Movie | 2021 | Self | Self - Host |
| Entertainment Tonight | 2021 | Self | Self |
| Late Night with Seth Meyers | 2021 | Self | Self - Guest |
| Today | 2021 | Self | Self - Guest |
| The Ellen DeGeneres Show | 2021 | Self | Self |
| Conan | 2020 | Self | Self |
| Who Wants $2.69? with Martin Urbano TV Series | 2020–2021 | Self | Self - Contestant |
| The Endless Honeymoon Podcast | 2020 | Self | Self - Guest Comedian |
| WGN Morning News | 2019 | Self | Self |
| Comedians in Cars Getting Coffee | 2019 | Self | Self |
| Laugh Factory | 2019 | Self | Self |
| The 4th Annual Howie Mandel Stand-Up Extravaganza | 2018 | Self | Self |
| Back to School Just for Laughs | 2018 | Self | Self |
| Night Train with Wyatt Cenac | 2018 | Self | Self |
| Hiking with Kevin | 2017 | Self | Self - Guest |
| @midnight | 2017 | Self | Self (as Melissa Villasenor) |
| The Night Time Show | 2016 | Self | Self - Guest |
| First Impressions with Dana Carvey | 2016 | Self | Self - Contestant (as Melissa Villasenor) |
| The Night Time Show with Stephen Kramer Glickman | 2016 | Self | Self (as Melissa Villasenor) |
| Richard Rossi Live TV Series | 2016 | Self | Self (as Melissa Villasenor) |
| What's Up Fool? Podcast Podcast Series | 2015 | Self | Self - Guest |
| Running Lines with Lenoci TV Series | 2014 | Self | Self (as Melissa Villasenor) |
| Talking Marriage with Ryan Bailey | 2014 | Self | Self (as Melissa Villasenor) |
| Picture Day TV Mini Series | 2014 | Self | Self (as Melissa Villasenor) |
| Access Hollywood | 2014 | Self | Self (as Melissa Villasenor) |
| All Growz Up with Melinda Hill TV Series | 2013 | Self | Self (as Melissa Villasenor) |
| Monkey Radio with Marc: The Animated Series TV Series | 2013 | Self | Self (as Melissa Villasenor) |
| Just for Laughs: All-Access | 2012 | Self | Self |
| Funny Girl Short | 2012 | Self | Self (as Melissa Villasenor) |
| StandUp in Stilettos | 2012 | Self | Self - performer (as Melissa Villasenor) |
| Blouze-O-Ween Live 3D TV Special | 2012 | Self | Self |
| Funny as Hell | 2012 | Self | Self - Comedian (as Melissa Villasenor) |
| America's Got Talent | 2011 | Self | Self - Contestant |
| Latino 101 | 2010–2011 | Self | Self (as Melissa Villasenor) |
| SNL50: Beyond Saturday Night | 2025 | Archive Footage | Self - Cast Member 2016 - 2022 (archive footage) |
| Velshi | 2021 | Archive Footage | Alexandria Ocasio-Cortez (archive footage) |
| Morning Joe | 2021 | Archive Footage | Self (archive footage) |
| MechaRandom42 | 2021 | Archive Footage | Self (archive footage) |
| Saturday Night Live Election Special 2020 TV Special | 2020 | Archive Footage | Impeachment Town Hall audience member |
| A Saturday Night Live Mother's Day | 2020 | Archive Footage | Amy (archive footage, uncredited) |
| Saturday Night Live Christmas Special | 2019 | Archive Footage | Christmas Candle receiver |